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1无需电极接触（避免了电极接触不良）
2无需荧光标记
3无耗材，商业96孔板直接使用（节省大量后续运行成本）
4自带温度控制器，无需其他温控设备等
Just place a culture plate on this instrument!
Activity of cardiomyocytes in a culture plate can be measured without staining and invasion. Result is displayed in real time in a waveform, and number of beats and contraction time are automatically detected with a single button. Built-in temperature control and plate moving mechanism. It is small and lightweight making it ideal for personal use.
Easy Operation
Data Example
Measurement results of cultured cardiomyocytes
bpm: Number of beats per minute
Ti: Cycle time of waveform
Td: Width of waveform (Approximately 80%)
Tpk: Time from rise of waveform to peak
Size: Height from baseline to peak (16-bit signed integer)
Applications
・Count of number of beats of cultured cardiomyocytes
・Confirmation of differentiation of iPS-derived cardiomyocytes
・Cardiotoxicity assay for new drug candidates
・Simple measurement of membrane potential using voltage-sensitive dye
Method and principle of Measurement
The principle of measurement of activities is quite simple.
It is well known that cell activities change light scattering, and beating cardiomyocytes show larger changes by beating activities.
LED under cultureware illuminates sample at low angle. The scattered light reflected from cells is detected by photo-diode, then only change component is amplified and extracted as activity signal.
The extracted signal is digitized and amplified thousands of times, or tens of thousands of times. Then, cardiac activities are recognized by numeral process.
Structure of CIOS8
・All of light source, photo-detector, signal processing circuit, temperature control stage are built in main
unit
・Motorized well-plate carrier
3 rows can be measured at same time. Since well-plate is moved right and left by the motorized well-
plate carrier , 96 wells can be measured (8-wells x 3 rows x 4 ).
This allows to measure cardiomyocytes activity under stable conditions without opening the lid.
Specifications
| Product name | Cardio Intrinsic Optical Signal Acquisition System CIOS8 |
|---|---|
| Model | CIOS8 |
| System configuration | CIOS8, USB2.0 cable, AC adaptor (x2), Control software |
| Available plate |
Culture plate with 96-wells or less (Any manufacturer's culture plate can be used) |
| Mechanism for plate movement | Electrical carrier |
| Temperature control | Automatic |
| Detectable item | Number of heart beat, Contraction time |
| Interface | USB2.0 |
| Control PC | Windows 7/10 64bit Recommended |
| Recommended operating condition | Temperature range: 20℃ - 30℃, Humidity range: 30% - 80% |
| Dimension | 185mm (W) x 115mm (D) x 75mm (H) |
| Weight | Approx. 1kg |
| Power supply | AD24V1A (x2) |
* Specifications & appearance are subject to change without prior notice due to continuous improvements.
* All products are made in Japan.
Option
・Simple measurement of membrane potential using voltage-sensitive dye
