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Overview
WI6X is a water immersion objective lens specialized for high-speed fluorescence imaging applications such as voltage-sensitive dye imaging and calcium imaging etc., which require the detection of very small changes in fluorescence of biological samples. Features include low magnification (3x-8x), high numerical aperture (0.35), and relatively long working distance (6mm).
Generally, the main reasons for designing a low magnification objective lens are to reduce lens distortion and create a long focal depth, but consequently these lenses also have low numerical apertures. However, an objective lens with a high numerical aperture is essential to reduce noise and optimize S/N ratios for high-speed fluorescence imaging.
Light reflection and perfusion or water flow on the surface of biological solutions are also sources of noises for high-speed imaging. Water immersion objectives eliminate these causes of noise significantly. Brainvision has designed a unique water immersion objective lens based on these requirements for biological imaging. The lens has a relatively long working distance of 6mm which allows users to easily position a microelectrode to biological preparations.
Specifications
Product Name | WI6X |
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Magnification | On THT-microscope: 2.5 - 6.7X with 1.6X condensing lens: 2.5X with 1X condensing lens: 4X with 0.63X condensing lens: 6.7X On Olympus BX51WI microscope: 9.2X (Slide revolver "U-SLRE" or equivalent is needed.) |
Focal Length | 20.0mm |
Numerical Aperture | 0.35 |
Field Diameter | φ3mm |
Wave Length | 450 - 700nm |
Working Distance | 6mm |
Adaptable Microscope | Brainvision: THT-microscope Olympus: BX51WI or others Nikon, Zeiss, Leica or the others: Ask |